Biofunctional Paper via the Covalent Modification of Cellulose

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• 作者：Arthur Yu ; Jing Shang ; Fang Cheng ; Bradford A. Paik ; Justin M. Kaplan ; Rodrigo B. Andrade ; Daniel M. Ratner
• 刊名：Langmuir
• 出版年：2012
• 出版时间：July 31, 2012
• 年：2012
• 卷：28
• 期：30
• 页码：11265-11273
• 全文大小：418K
• 年卷期：v.28,no.30(July 31, 2012)
• ISSN：1520-5827

文摘

Paper-based analytical devices are the subject of growing interest for the development of low-cost point-of-care diagnostics, environmental monitoring technologies, and research tools for limited-resource settings. However, there are limited chemistries available for the conjugation of biomolecules to cellulose for use in biomedical applications. Herein, divinyl sulfone (DVS) chemistry was demonstrated to immobilize small molecules, proteins, and DNA covalently onto the hydroxyl groups of cellulose membranes through nucleophilic addition. Assays on modified cellulose using protein鈥揷arbohydrate and protein鈥揼lycoprotein interactions as well as oligonucleotide hybridization showed that the membrane鈥檚 bioactivity was specific, dose-dependent, and stable over a long period of time. The use of an inkjet printer to form patterns of biomolecules on DVS-activated cellulose illustrates the adaptability of the DVS functionalization technique to pattern sophisticated designs, with potential applications in cellulose-based lateral flow devices.