Facile Hydrothermal Synthesis and Surface Functionalization of Polyethyleneimine-Coated Iron Oxide Nanoparticles for Biomedical Applications

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• 作者：Hongdong Cai ; Xiao An ; Jun Cui ; Jingchao Li ; Shihui Wen ; Kangan Li ; Mingwu Shen ; Linfeng Zheng ; Guixiang Zhang ; Xiangyang Shi
• 刊名：ACS Applied Materials & Interfaces
• 出版年：2013
• 出版时间：March 13, 2013
• 年：2013
• 卷：5
• 期：5
• 页码：1722-1731
• 全文大小：525K
• 年卷期：v.5,no.5(March 13, 2013)
• ISSN：1944-8252

文摘

We report the facile hydrothermal synthesis and surface functionalization of branched polyethyleneimine (PEI)-coated iron oxide nanoparticles (Fe₃O₄鈥揚EI NPs) for biomedical applications. In this study, Fe₃O₄鈥揚EI NPs were synthesized via a one-pot hydrothermal method in the presence of PEI. The formed Fe₃O₄鈥揚EI NPs with primary amine groups on the surface were able to be further functionalized with polyethylene glycol (PEG), acetic anhydride, and succinic anhydride, respectively. The formed pristine and functionalized Fe₃O₄鈥揚EI NPs were characterized via different techniques. We showed that the sizes of the Fe₃O₄鈥揚EI NPs were able to be controlled by varying the mass ratio of Fe(II) salt and PEI. In addition, the formed Fe₃O₄鈥揚EI NPs with different surface functionalities had good water dispersibility, colloidal stability, and relatively high R₂ relaxivity (130鈥?60 1/(mM路s)). Cell viability assay data revealed that the surface PEGylation and acylation of Fe₃O₄鈥揚EI NPs rendered them with good biocompatibility in the given concentration range, while the pristine aminated Fe₃O₄鈥揚EI NPs started to display slight toxicity at the concentration of 50 渭g/mL. Importantly, macrophage cellular uptake results demonstrated that both PEGylation and acetylation of Fe₃O₄鈥揚EI NPs were able to significantly reduce the nonspecific macrophage uptake, likely rendering the particles with prolonged circulation time. With the proven hemocompatibility and rich amine conjugation chemistry, the Fe₃O₄鈥揚EI NPs with different surface functionalities may be applied for various biomedical applications, especially for magnetic resonance imaging and therapy.

Keywords:

iron oxide nanoparticles; polyethyleneimine; hydrothermal synthesis; biocompatibility; macrophage cellular uptake