Capillary Electrophoresis of Sialic Acid-Containing Glycoprotein. Effect of the Heterogeneity of Carbohydrate Chains on Glycoform Separation Using an 详细信息    查看全文

• 作者：Kazuaki Kakehi ; Mitsuhiro Kinoshita ; Daisuke Kawakami ; Junko Tanaka ; Kazuo Sei ; Kaori Endo ; Yasuo Oda ; Masahiro Iwaki ; and Takashi Masuko
• 刊名：Analytical Chemistry
• 出版年：2001
• 出版时间：June 1, 2001
• 年：2001
• 卷：73
• 期：11
• 页码：2640 - 2647
• 全文大小：105K
• 年卷期：v.73,no.11(June 1, 2001)
• ISSN：1520-6882

文摘

₁-Acid glycoprotein (AGP) showed multiple peaks onseparation using capillary electrophoresis in a chemicallymodified capillary with dimethylpolysiloxane at slightlyacidic conditions. We analyzed glycoforms of AGP speciesafter separation by ion-exchange chromatography, Con Aaffinity chromatography, and Cu(II)-chelating affinity chromatography. The AGP species thus obtained were digested with N-glycosidase F, and the released carbohydrate chains were analyzed by high-performance liquidchromatography after labeling with 3-aminobenzoic acid.The results afforded basic information on the contributionof carbohydrate chains to the separation mechanism ofglycoforms of AGP by capillary electrophoresis. In addition, we describe an easy method for AGP analysis inserum samples using the electrokinetic injection.