Suppression of γ-Tocotrienol on UVB Induced Inflammation in HaCaT Keratinocytes and HR-1 Hairless Mice via Inflammatory Mediators Multiple Signaling

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• 作者：Akira Shibata ; Kiyotaka Nakagawa ; Yuki Kawakami ; Tsuyoshi Tsuzuki ; Teruo Miyazawa
• 刊名：Journal of Agricultural and Food Chemistry
• 出版年：2010
• 出版时间：June 9, 2010
• 年：2010
• 卷：58
• 期：11
• 页码：7013-7020
• 全文大小：945K
• 年卷期：v.58,no.11(June 9, 2010)
• ISSN：1520-5118

文摘

Tocopherol (Toc) such as α-Toc has been expected to act as photochemopreventive agent of skin, but the effect of the other vitamin E forms [tocotrienols (T3)] has not been fully understood. We evaluated the anti-inflammatory effect of T3 on UVB-induced inflammatory reaction using immortalized human keratinocytes and hairless mice. γ-T3 suppressed UVB-induced PGE₂ production while similar α-Toc doses had no effect. The anti-inflammatory actions of γ-T3 were explained by its ability to reduce UVB-induced inflammatory gene and protein expression [cyclooxgenase-2 (COX-2), interleukin (IL)-1β, IL-6, and monocyte chemotactic protein-1]. Western blot analysis revealed γ-T3 inhibited p38, extracellular signal-regulated kinase, and c-Jun N-terminal kinase/stress-activated protein kinase activation. In HR-1 hairless mice, oral T3 suppressed UVB-induced changes in skin thickness, COX-2 protein expression, and hyperplasia, but α-Toc did not. These results suggest T3 has potential use to protect against UVB-induced skin inflammation.