Solution Structure of the DNA-Binding Domain of TraM

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• 作者：Thomas Stockner ; Carmela Plugariu ; Gü ; nther Koraimann ; Gregor Hö ; genauer ; Wolfgang Bermel ; Stefan Prytulla ; and Heinz Sterk
• 刊名：Biochemistry
• 出版年：2001
• 出版时间：March 20, 2001
• 年：2001
• 卷：40
• 期：11
• 页码：3370 - 3377
• 全文大小：293K
• 年卷期：v.40,no.11(March 20, 2001)
• ISSN：1520-4995

文摘

The solution structure of the DNA-binding domain of the TraM protein, an essential componentof the DNA transfer machinery of the conjugative resistance plasmid R1, is presented. The structure hasbeen determined using homonuclear 2-dimensional NMR spectroscopy as well as ¹⁵N labeled heteronuclear2- and 3-dimensional NMR spectroscopy. It turns out that the solution structure of the DNA bindingdomain of the TraM protein is globular and dominantly helical. The very first amino acids of the N-terminusare unstructured.