Identification of B-Form DNA in an Ultrahigh Vacuum by Noncontact-Mode Atomic Force Microscopy
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- 作者：Takayuki Uchihashi ; Masato Tanigawa ; Makoto Ashino ; Yasuhiro Sugawara ; Kousuke Yokoyama ; Seizo Morita ; and Mitsuru Ishikawa
- 刊名：Langmuir
- 出版年：2000
- 出版时间：February 8, 2000
- 年：2000
- 卷：16
- 期：3
- 页码：1349 - 1353
- 全文大小：182K
- 年卷期：v.16,no.3(February 8, 2000)
- ISSN：1520-5827
文摘
Double-stranded (ds) DNA was imaged by noncontact-mode atomic force microscopy in an ultrahighvacuum (UHV) after thermal annealing (<100 
C). The contour length of DNA measured (3300 Å) wasconsistent with that of the B-form DNA (1000 bp) we prepared. In addition, we resolved right-handedhelical turns with a spacing of 33 ± 2 Å. These observations showed both UHV condition and the thermalannealing did not make DNA take A-form but kept B-form.
C). The contour length of DNA measured (3300 Å) wasconsistent with that of the B-form DNA (1000 bp) we prepared. In addition, we resolved right-handedhelical turns with a spacing of 33 ± 2 Å. These observations showed both UHV condition and the thermalannealing did not make DNA take A-form but kept B-form.