Analysis of Oxidative DNA Damage 8-Hydroxy-2'-deoxyguanosine as a Biomarker of Exposures to Persistent Pollutants for Marine Mammals
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- 作者：Chi-Shan Li ; Kuen-Yuh Wu ; Gou-Ping Chang-Chien ; and Chin-Cheng Chou
- 刊名：Environmental Science & Technology
- 出版年：2005
- 出版时间：April 15, 2005
- 年：2005
- 卷：39
- 期：8
- 页码：2455 - 2460
- 全文大小：108K
- 年卷期：v.39,no.8(April 15, 2005)
- ISSN：1520-5851
文摘
An oxidative DNA biomarker, 8-hydroxy-2'-deoxyguanosine(8-OHdG), was determined in the livers and kidneys ofstranded or by-caught cetaceans along the Taiwan coastthrough isotope-dilution liquid chromatography coupledwith tandem mass spectrometry (LC/MS/MS) to evaluatethe feasibility of analyzing the DNA adduct in marine mammalsand then to study the association between 8-OHdG andlevels of polychlorinated biphenyls (PCBs) and dichlorodiphenyldichloroethylene (DDE) in the blubbers of thecetaceans. The mean values of the 8-OHdG from the liverand kidney samples were 19.83 ± 10.00 pmol/
moldeoxyguanosine (dG) (6.90-53.53 pmol/mol dG) and 19.16± 7.48 pmol/mol dG (5.36-39.36 pmol/mol dG),respectively. In general, 8-OHdG was not related to thegeneral health status of the by-caught and stranded animalsand also was not related to species. However, the levelsof 8-OHdG had a positive correlation with concentrations ofPCBs, but not DDE, in female cetacean livers. In addition,when selected coplanar PCBs (dioxin-like congeners)were used to compare the 2,3,7,8-tetrachlorodibenzo-p-dioxin equivalents (TEQs) with 8-OHdG of by-caught cetaceans,a high positive correlation (r = 0.80, p < 0.01) wasfound in mature female animals. Thus, the detection of8-OHdG in marine mammals with isotope-dilution LC/MS/MS is possible, and the study of the relationship betweenoxidative DNA damage and environmental contaminantsunder natural exposure indicates that the level of 8-OHdGin female cetacean livers is associated with coplanarPCBs and the factor of sexual maturity.
moldeoxyguanosine (dG) (6.90-53.53 pmol/mol dG) and 19.16± 7.48 pmol/mol dG (5.36-39.36 pmol/mol dG),respectively. In general, 8-OHdG was not related to thegeneral health status of the by-caught and stranded animalsand also was not related to species. However, the levelsof 8-OHdG had a positive correlation with concentrations ofPCBs, but not DDE, in female cetacean livers. In addition,when selected coplanar PCBs (dioxin-like congeners)were used to compare the 2,3,7,8-tetrachlorodibenzo-p-dioxin equivalents (TEQs) with 8-OHdG of by-caught cetaceans,a high positive correlation (r = 0.80, p < 0.01) wasfound in mature female animals. Thus, the detection of8-OHdG in marine mammals with isotope-dilution LC/MS/MS is possible, and the study of the relationship betweenoxidative DNA damage and environmental contaminantsunder natural exposure indicates that the level of 8-OHdGin female cetacean livers is associated with coplanarPCBs and the factor of sexual maturity.