Multiplex Targeting, Tracking, and Imaging of Apoptosis by Fluorescent Surface Enhanced Raman Spectroscopic Dots
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- 作者：Kyeong Nam Yu ; Sang-Myung Lee ; Ji Yun Han ; Hyunmi Park ; Min-Ah Woo ; Mi Suk Noh ; Soon-Kyung Hwang ; Jung-Taek Kwon ; Hua Jin ; Yong-Kweon Kim ; Paul J. Hergenrother ; Dae Hong Jeong ; Yoon-Sik Lee ; Myung-Hain
- 刊名：Bioconjugate Chemistry
- 出版年：2007
- 出版时间：July 2007
- 年：2007
- 卷：18
- 期：4
- 页码：1155 - 1162
- 全文大小：960K
- 年卷期：v.18,no.4(July 2007)
- ISSN：1520-4812
文摘
We have developed multifunctional fluorescent surface enhanced Raman spectroscopic tagging material (F-SERSdots) composed of silver nanoparticle-embedded silica spheres with fluorescent organic dye and specific Ramanlabels for multiplex targeting, tracking, and imaging of cellular/molecular events in the living organism. In thisstudy, F-SERS dots fabricated with specific target antibodies (BAX and BAD) were employed for the detectionof apoptosis. The F-SERS dots did not show any particular toxicity in several cell lines. The F-SERS dots couldmonitor the apoptosis effectively and simultaneously through fluorescent images as well as Raman signals inboth cells and tissues with high selectivity. Our results clearly demonstrate that F-SERS dots can be easily applicableto multiplex analysis of diverse cellular/molecular events important for maintaining cellular homeostasis.