Detection of Membrane Biointeractions Based on Fluorescence Superquenching

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• 作者：Reema Zeineldin ; Menake E. Piyasena ; Larry A. Sklar ; David Whitten ; Gabriel P. Lopez
• 刊名：Langmuir
• 出版年：2008
• 出版时间：April 15, 2008
• 年：2008
• 卷：24
• 期：8
• 页码：4125 - 4131
• 全文大小：210K
• 年卷期：v.24,no.8(April 15, 2008)
• ISSN：1520-5827

文摘

Assays for biointeractions of molecules with supported lipid bilayers using fluorescence superquenching are described.A conjugated cationic polymer was adsorbed on to silica microspheres, which were then coated with an anionic lipidbilayer. The lipid bilayer attenuated superquenching by acting as a barrier between the conjugated polymer and itsquencher. Biointeractions of the lipid bilayer with a membrane lytic peptide, melittin, were detected and quantitatedby superquenching of the conjugated polyelectrolyte in flow cytometric and microfluidic bioassays. A higher sensitivityfor detecting melittin lysis of the lipid bilayer at lower concentrations and shorter times for melittin action was foundusing flow cytometry in this study in comparison to other existing methods. This study combined the sensitivity ofsuperquenching and flow cytometry to detect biointeractions with a lipid bilayer, which serves as a platform fordeveloping functional assays for sensor applications, lipid enzymology, and investigations of molecular interactions.In addition, this study demonstrated proof-of-concept for using superquenching detected as a result of lipid bilayerdisruption in a microfluidic format.