Real-Time Detection of Telomerase Activity Using the Exponential Isothermal Amplification of Telomere Repeat Assay

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• 作者：Leilei Tian ; Yossi Weizmann
• 刊名：The Journal of the American Chemical Society
• 出版年：2013
• 出版时间：February 6, 2013
• 年：2013
• 卷：135
• 期：5
• 页码：1661-1664
• 全文大小：250K
• 年卷期：v.135,no.5(February 6, 2013)
• ISSN：1520-5126

文摘

As crucial pieces in the puzzle of cancer and human aging, telomeres and telomerase are indispensable in modern biology. Here we describe a novel exponential isothermal amplification of telomere repeat (EXPIATR) assay鈥攁 sensitive, simple, and reliable in vitro method for measuring telomerase activity in cell extracts. Through a strategically designed path of nucleic acid isothermal amplifications, EXPIATR abandons the expensive thermal cycling protocol and achieves ultrafast detection: telomerase activity equivalent to a single HeLa cancer cell can be detected in 25 min.