Inorganic Phosphate and Nucleotides on Silica Surface: Condensation, Dismutation, and Phosphorylation

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• 作者：Thomas Georgelin ; Maguy Jaber ; Thomas Onfroy ; Aaron-Albert Hargrove ; France Costa-Torro ; Jean-Francois Lambert
• 刊名：The Journal of Physical Chemistry C
• 出版年：2013
• 出版时间：June 20, 2013
• 年：2013
• 卷：117
• 期：24
• 页码：12579-12590
• 全文大小：359K
• 年卷期：v.117,no.24(June 20, 2013)
• ISSN：1932-7455

文摘

We explore the reactivity of inorganic monophosphate ions (Pi) and 5鈥?adenosine monophosphate (AMP), adsorbed onto amorphous silica separately or together. This question has relevance for prebiotic chemistry scenarios and, more generally, for biomedical applications involving biomolecule adsorption. XRD, TGA, and ³¹P and ²⁹Si NMR results show that inorganic phosphate ions deposited on silica condense to polyphosphates at considerably lower temperatures than in bulk KH₂PO₄. In the same temperature range, AMP adsorbed alone undergoes dismutation reactions, yielding adenosine, ADP, and ATP; in this case, the effect of the silica surface is not obvious. When AMP and Pi are coadsorbed on silica at high loadings (5鈥?0%), AMP dismutation and phosphorylation by Pi both occur, allowing the formation of ADP and ATP. The latter result clearly shows the ability of silica surfaces to promote the formation of molecules generally considered as 鈥渉igh-energy鈥?compounds and opens the way to further research on the effect of mineral surfaces for nucleotide synthesis and ribose stabilization.