Electrospray Characteristic Curves: In Pursuit of Improved Performance in the Nanoflow Regime
详细信息 查看全文
- 作者：Ioan Marginean ; Ryan T. Kelly ; Jason S. Page ; Keqi Tang ; Richard D. Smith
- 刊名：Analytical Chemistry
- 出版年：2007
- 出版时间：November 1, 2007
- 年：2007
- 卷：79
- 期：21
- 页码：8030 - 8036
- 全文大小：320K
- 年卷期：v.79,no.21(November 1, 2007)
- ISSN：1520-6882
文摘
Depending on its coordinates in the parameter space, anelectrospray can manifest in one of several knownregimes-stable, quasi-stable, transitional chaotic, andnonaxial-that ultimately impact measurement sensitivityand precision. An electrospray operating in the cone-jetregime provides relatively large and stable spray current,as well as smaller initial droplets, that are prerequisitesfor higher sensitivity and quality mass spectrometricanalyses. However, the dynamic conditions encountered,for example, in gradient elution-based liquid separationscreate difficulties for continuous operation in this regime.We present a preliminary study aimed at providing thebasis for stabilizing the electrospray in the cone-jet regime.On the basis of spray current measurements obtainedusing solvent conditions typically found in liquid chromatography-mass spectrometry, an improved descriptionof the cone-jet stability island is provided by includingtransitions to and from the recently described astableregime. Additionally, the experimental conditions in whichthe astable regime marks the transition between pulsatingand cone-jet regimes are further clarified.