Identification of Ah Receptor Agonists in Sediment of Meiliang Bay, Taihu Lake, China
详细信息 查看全文
- 作者：Min Qiao ; Yingying Chen ; Qinghua Zhang ; Shengbiao Huang ; Mei Ma ; Chunxia Wang ; and Zijian Wang
- 刊名：Environmental Science & Technology
- 出版年：2006
- 出版时间：March 1, 2006
- 年：2006
- 卷：40
- 期：5
- 页码：1415 - 1419
- 全文大小：110K
- 年卷期：v.40,no.5(March 1, 2006)
- ISSN：1520-5851
文摘
Deterioration of ecosystem and water quality in TaihuLake, the third largest freshwater lake in China, is of greatconcern in China. However, most evaluations on waterand sediment were carried out by chemical analysis, whichcould hardly gain information on the risk stressorsaffecting the system. Sediment samples from MeiliangBay, Taihu Lake were assayed for AhR-mediated ERODinduction using a rat hepatoma cell line (H4IIE). The knownAhR agonists including PCDD/Fs, PCBs, and PAHs wereanalyzed by instruments. The cause-effect relationshipbetween the observed EROD activity and chemicalconcentrations of AhR agonists and their dose-effectrelationship were examined. Our results showed that sedimentextracts could induce significant AhR effects, and thechemical-derived TCDD equivalents (TEQcal) were significantlycorrelated to bioassay-derived TCDD equivalents (TEQbio)(R = 0.85, p < 0.01). Among different contributors, PAHscould account for 70-93% and PCDD/Fs could contributeless than 30% of TEQcal. PCBs exhibited almost no contribution.Therefore, integration of EROD bioassay and chemicalanalysis provided an effective way for identifying the potentialrisk stressors. Under this circumstance, a quantitative dose-effect relationship between TEQPAH and EROD activitycould be established, suggesting that the observed AhReffect was mostly derived from PAHs. The implication is thatthe EROD assay and the identified risk stressors (PAHs)may be used in lieu of detailed environmental analysis tofollow the progression of a remediation strategy.