1-Methylthio-d-xylulose 5-Phosphate Methylsulfurylase: A Novel Route to 1-Deoxy-d-xylulose 5-Phosphate in Rhodospirillum rubrum

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• 作者：Benjamin P. Warlick ; Bradley S. Evans ; Tobias J. Erb ; Udipi A. Ramagopal ; Jaya Sriram ; Heidi J. Imker ; J. Michael Sauder ; Jeffrey B. Bonanno ; Stephen K. Burley ; F. Robert Tabita ; Steven C. Almo ; Jonathan S. Sweedler ; John A. Gerlt
• 刊名：Biochemistry
• 出版年：2012
• 出版时间：October 23, 2012
• 年：2012
• 卷：51
• 期：42
• 页码：8324-8326
• 全文大小：250K
• 年卷期：v.51,no.42(October 23, 2012)
• ISSN：1520-4995

文摘

Rhodospirillum rubrum produces 5-methylthioadenosine (MTA) from S-adenosylmethionine in polyamine biosynthesis; however, R. rubrum lacks the classical methionine salvage pathway. Instead, MTA is converted to 5-methylthio-d-ribose 1-phosphate (MTR 1-P) and adenine; MTR 1-P is isomerized to 1-methylthio-d-xylulose 5-phosphate (MTXu 5-P) and reductively dethiomethylated to 1-deoxy-d-xylulose 5-phosphate (DXP), an intermediate in the nonmevalonate isoprenoid pathway [Erb, T. J., et al. (2012) Nat. Chem. Biol., in press]. Dethiomethylation, a novel route to DXP, is catalyzed by MTXu 5-P methylsulfurylase. An active site Cys displaces the enolate of DXP from MTXu 5-P, generating a methyl disulfide intermediate.