Novel Parallelized Quadrupole/Linear Ion Trap/Orbitrap Tribrid Mass Spectrometer Improving Proteome Coverage and Peptide Identification Rates

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• 作者：Michael W. Senko ; Philip M. Remes ; Jesse D. Canterbury ; Raman Mathur ; Qingyu Song ; Shannon M. Eliuk ; Chris Mullen ; Lee Earley ; Mark Hardman ; Justin D. Blethrow ; Huy Bui ; August Specht ; Oliver Lange ; Eduard Denisov ; Alexander Makarov ; Stevan Horning ; Vlad Zabrouskov
• 刊名：Analytical Chemistry
• 出版年：2013
• 出版时间：December 17, 2013
• 年：2013
• 卷：85
• 期：24
• 页码：11710-11714
• 全文大小：269K
• 年卷期：v.85,no.24(December 17, 2013)
• ISSN：1520-6882

文摘

Proteome coverage and peptide identification rates have historically advanced in line with improvements to the detection limits and acquisition rate of the mass spectrometer. For a linear ion trap/Orbitrap hybrid, the acquisition rate has been limited primarily by the duration of the ion accumulation and analysis steps. It is shown here that the spectral acquisition rate can be significantly improved through extensive parallelization of the acquisition process using a novel mass spectrometer incorporating quadrupole, Orbitrap, and linear trap analyzers. Further, these improvements to the acquisition rate continue to enhance proteome coverage and general experimental throughput.