Nanometer Distance Measurements between Multicolor Quantum Dots

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• 作者：Josh Antelman ; Connie Wilking-Chang ; Shimon Weiss ; Xavier Michalet
• 刊名：Nano Letters
• 出版年：2009
• 出版时间：May 13, 2009
• 年：2009
• 卷：9
• 期：5
• 页码：2199-2205
• 全文大小：302K
• 年卷期：v.9,no.5(May 13, 2009)
• ISSN：1530-6992

文摘

Quantum dot dimers made of short double-stranded DNA molecules labeled with different color quantum dots at each end were imaged using multicolor stage-scanning confocal microscopy. This approach eliminates chromatic aberration and color registration issues usually encountered in other multicolor imaging techniques. We demonstrate nanometer accuracy in individual distance measurement by suppression of quantum dot blinking and thoroughly characterize the contribution of different effects to the variability observed between measurements. Our analysis opens the way to accurate structural studies of biomolecules and biomolecular complexes using multicolor quantum labeling.