Effective Brownian Ratchet Separation by a Combination of Molecular Filtering and a Self-Spreading Lipid Bilayer System

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• 作者：Toshinori Motegi ; Hideki Nabika ; Yingqiang Fu ; Lili Chen ; Yinlu Sun ; Jianwei Zhao ; Kei Murakoshi
• 刊名：Langmuir
• 出版年：2014
• 出版时间：July 1, 2014
• 年：2014
• 卷：30
• 期：25
• 页码：7496-7501
• 全文大小：362K
• ISSN：1520-5827

文摘

A new molecular manipulation method in the self-spreading lipid bilayer membrane by combining Brownian ratchet and molecular filtering effects is reported. The newly designed ratchet obstacle was developed to effectively separate dye鈥搇ipid molecules. The self-spreading lipid bilayer acted as both a molecular transport system and a manipulation medium. By controlling the size and shape of ratchet obstacles, we achieved a significant increase in the separation angle for dye鈥搇ipid molecules compared to that with the previous ratchet obstacle. A clear difference was observed between the experimental results and the simple random walk simulation that takes into consideration only the geometrical effect of the ratchet obstacles. This difference was explained by considering an obstacle-dependent local decrease in molecular diffusivity near the obstacles, known as the molecular filtering effect at nanospace. Our experimental findings open up a novel controlling factor in the Brownian ratchet manipulation that allow the efficient separation of molecules in the lipid bilayer based on the combination of Brownian ratchet and molecular filtering effects.