Increased -Carotene Production in Recombinant Escherichia coli Harboring an Engineered Isoprenoid Precursor Pa
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- 作者：Sang-Hwal Yoon ; Hye-Min Park ; Ju-Eun Kim ; Sook-Hee Lee ; Myung-Suk Choi ; Jae-Yean Kim ; Deok-Kun Oh ; Jay D. Keasling ; Seon-Won Kim
- 刊名：Biotechnology Progress
- 出版年：2007
- 出版时间：June 2007
- 年：2007
- 卷：23
- 期：3
- 页码：599 - 605
- 全文大小：99K
- 年卷期：v.23,no.3(June 2007)
- ISSN：1520-6033
文摘
When pT-LYCm4 containing lycopene synthetic genes was co-transformed with pSUcrtY orpSHcrtY containing crtY gene of Pantoea ananatis (P. ananatis) or Pantoea agglomerans (P.agglomerans), 
-carotene productions of 36 and 35 mg/L were obtained, respectively. Nolycopene was detected in the -carotene production culture. pT-HB, constructed by addition ofP. ananatis crtY gene into pT-LYCm4, was used for co-transformation with pSdxs andpSSN12Didi, which increased isopentenyl diphosphate and dimethylallyl diphosphate synthesis.-Carotene production significantly increased 1.5-fold (51 mg/L) with the amplification of thedxs gene through pSdxs and 4-fold (135 mg/L) with the mevalonate bottom pathway ofpSSN12Didi in the presence of 3.3 mM mevalonate. The pT-DHB, constructed by integratingthe dxs gene into pT-HB, was used for cotransformation of Escherichia coli (E. coli) harboringpSSN12Didi, resulting in -carotene production of 141 mg/L. Recombinant E. coli harboringpT-DHB and pSSN12Didi was used to maximize -carotene production by adjusting the availableamounts of glycerol, a carbon source, and mevalonate, the precursor of the mevalonate bottompathway. When recombinant E. coli was given 16.5 mM mevalonate and 2.5% (w/v) glycerol,-carotene production of 503 mg/L in concentration and 49.3 mg/g DCW in content was obtainedat 144 h, which was the highest level of carotenoid production in E. coli ever reported in theliterature.
-carotene productions of 36 and 35 mg/L were obtained, respectively. Nolycopene was detected in the -carotene production culture. pT-HB, constructed by addition ofP. ananatis crtY gene into pT-LYCm4, was used for co-transformation with pSdxs andpSSN12Didi, which increased isopentenyl diphosphate and dimethylallyl diphosphate synthesis.-Carotene production significantly increased 1.5-fold (51 mg/L) with the amplification of thedxs gene through pSdxs and 4-fold (135 mg/L) with the mevalonate bottom pathway ofpSSN12Didi in the presence of 3.3 mM mevalonate. The pT-DHB, constructed by integratingthe dxs gene into pT-HB, was used for cotransformation of Escherichia coli (E. coli) harboringpSSN12Didi, resulting in -carotene production of 141 mg/L. Recombinant E. coli harboringpT-DHB and pSSN12Didi was used to maximize -carotene production by adjusting the availableamounts of glycerol, a carbon source, and mevalonate, the precursor of the mevalonate bottompathway. When recombinant E. coli was given 16.5 mM mevalonate and 2.5% (w/v) glycerol,-carotene production of 503 mg/L in concentration and 49.3 mg/g DCW in content was obtainedat 144 h, which was the highest level of carotenoid production in E. coli ever reported in theliterature.