Enhanced Vanillin Production from Recombinant E. coli Using NTG Mutagenesis and Adsorbent Resin

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• 作者：Sang-Hwal Yoon ; Eun-Gyeong Lee ; Amitabha Das ; Sook-Hee Lee ; Cui Li ; Hee-Kyoung Ryu ; Myung-Suk Choi ; Weon-Taek Seo ; Seon-Won Kim
• 刊名：Biotechnology Progress
• 出版年：2007
• 出版时间：October 2007
• 年：2007
• 卷：23
• 期：5
• 页码：1143 - 1148
• 全文大小：184K
• 年卷期：v.23,no.5(October 2007)
• ISSN：1520-6033

文摘

Vanillin production was tested with different concentrations of added ferulic acid in E. coliharboring plasmid pTAHEF containing fcs (feruloyl-CoA synthase) and ech (enoyl-CoAhydratase/aldolase) genes cloned from Amycolatopsis sp. strain HR104. The maximum productionof vanillin from E. coli DH5 harboring pTAHEF was found to be 1.0 g/L at 2.0 g/L of ferulicacid for 48 h of culture. To improve the vanillin production by reducing its toxicity, twoapproaches were followed: (1) generation of vanillin-resistant mutant of NTG-VR1 throughNTG mutagenesis and (2) removal of toxic vanillin from the medium by XAD-2 resin absorption.The vanillin production of NTG-VR1 increased to three times at 5 g/L of ferulic acid whencompared with its wild-type strain. When 50% (w/v) of XAD-2 resin was employed in culturewith 10 g/L of ferulic acid, the vanillin production of NTG-VR1 was 2.9 g/L, which was 2-foldhigher than that obtained with no use of the resin.