Bioconjugation of l-3,4-Dihydroxyphenylalanine Containing Protein with a Polysaccharide

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• 作者：Niraikulam Ayyadurai ; Nadarajan Saravanan Prabhu ; Kanagavel Deepankumar ; Yoon Jung Jang ; Nataraj Chitrapriya ; Eunjung Song ; Nahum Lee ; Seog K. Kim ; Byung-Gee Kim ; Nagasundarapandian Soundrarajan ; Sungu Lee ; Hyung Joon Cha ; Nediljko Budisa ; Hyungdon Yun
• 刊名：Bioconjugate Chemistry
• 出版年：2011
• 出版时间：April 20, 2011
• 年：2011
• 卷：22
• 期：4
• 页码：551-555
• 全文大小：764K
• 年卷期：v.22,no.4(April 20, 2011)
• ISSN：1520-4812

文摘

We describe the simple bioconjugation strategy in combination of periodate chemistry and unnatural amino acid incorporation. The residue specific incorporation of 3,4-dihydroxy-l-phenylalanine can alter the properties of protein to conjugate into the polymers. The homogeneously modified protein will yield quinone residues that are covalently conjugated to nucleophilic groups of the amino polysaccharide. This novel approach holds great promise for widespread use to prepare protein conjugates and synthetic biology applications.