A Multidisciplinary Approach to Probing Enthalpy鈥揈ntropy Compensation and the Interfacial Mobility Model

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• 作者：Erin M. Wilfong ; Yuri Kogiso ; Sivaramakrishnan Muthukrishnan ; Thomas Kowatz ; Yu Du ; Amber Bowie ; James H. Naismith ; Christopher M. Hadad ; Eric J. Toone ; Terry L. Gustafson
• 刊名：Journal of the American Chemical Society
• 出版年：2011
• 出版时间：August 3, 2011
• 年：2011
• 卷：133
• 期：30
• 页码：11515-11523
• 全文大小：1097K
• 年卷期：v.133,no.30(August 3, 2011)
• ISSN：1520-5126

文摘

In recent years, interfacial mobility has gained popularity as a model with which to rationalize both affinity in ligand binding and the often observed phenomenon of enthalpy鈥揺ntropy compensation. While protein contraction and reduced mobility, as demonstrated by computational and NMR techniques respectively, have been correlated to entropies of binding for a variety of systems, to our knowledge, Raman difference spectroscopy has never been included in these analyses. Here, nonresonance Raman difference spectroscopy, isothermal titration calorimetry, and X-ray crystallography were utilized to correlate protein contraction, as demonstrated by an increase in protein interior packing and decreased residual protein movement, with trends of enthalpy鈥揺ntropy compensation. These results are in accord with the interfacial mobility model and lend additional credence to this view of protein activity.