In this paper, glycosaminoglycans from the body of the large freshwater mollusc bivalve
Anodonta anodontawere recovered at about 0.6 mg/g of dry tissue, composed of chondroitin sulfate (approximately 38%),nonsulfated chondroitin (about 21%), and heparin (41%). This last polysaccharide was found to consist ofa large percentage (approximately 88%) of a fast-moving species possessing a lower molecular mass andsulfate group amount and about 12% of a more sulfated, slow-moving component having a greater molecularmass. The chondroitin sulfate was composed of approximately 28% of the 6-sulfated disaccharide, 46% ofthe 4-sulfated disaccharide, and about 26% of the nonsulfated disaccharide, with a charge density value of0.74. Heparin was subjected to the oligosaccharide mapping after treatment with heparinase and then separationof the resulting unsaturated oligosaccharides by SAX-HPLC. A heparin sample from
Anodonta anodontashowed a degree of sulfation similar to that of bovine mucosal heparin because of the presence ofapproximately the same mol % of the trisulfated disaccharide (
UA2S(1
4)-
-
D-GlcN2S6S), a slightmodification of the other oligosaccharides, and a significant increase of the disaccharide bearing the sulfategroup in position 3 of the
N-sulfoglucosamine 6-sulfate (
4)-
-
D-GlcA(1
4)-
-
D-GlcN2S3S6S(1
) partof the ATIII-binding region. However, the anticoagulant activity of mollusc heparin was quite similar tothat of pharmaceutical grade heparin. The data obtained again emphasize the heterogeneity of GAGs frommolluscs.