Automated Affinity Capture-Release of Biotin-Containing Conjugates Using a Lab-on-Valve Apparatus Coupled to UV/Visible and Electrospray Ionization Mass Spectrometry
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- 作者：Yuko Ogata ; Louis Scampavia ; Jaromí ; r R
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- 刊名：Analytical Chemistry
- 出版年：2002
- 出版时间：September 15, 2002
- 年：2002
- 卷：74
- 期：18
- 页码：4702 - 4708
- 全文大小：138K
- 年卷期：v.74,no.18(September 15, 2002)
- ISSN：1520-6882
文摘
We report a new method for automated affinity captureand release of biotin-containing conjugates on immobilized streptavidin using a lab-on-valve (LOV) bead injection apparatus. The apparatus is also coupled to UV/visible and electrospray ionization mass spectrometry(ESI-MS) for monitoring the captured and released biotin-containing conjugates. Dissociation rate constants forrelease from streptavidin of two chromophore-taggedbiotin conjugates were measured by UV/visible spectrometry and the dissociation was simultaneously monitoredby ESI-MS. The LOV-ESI-MS instrument was also usedfor repetitive assays of lysosomal 
-galactosidase in human cell homogenates. Fast analysis in 4.5 min/full cycleand robust operation in 60 repetitive analyses are demonstrated that are promising for transfer of the LOV-ESI-MS technology into clinical practice.
-galactosidase in human cell homogenates. Fast analysis in 4.5 min/full cycleand robust operation in 60 repetitive analyses are demonstrated that are promising for transfer of the LOV-ESI-MS technology into clinical practice.