Exploiting Cascade Reactions in Bienzyme Layer-by-Layer Films

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• 作者：Rafael Furlan de Oliveira ; Marli Leite de Moraes ; Osvaldo N. Oliveira ; Jr. ; Marystela Ferreira
• 刊名：Journal of Physical Chemistry C
• 出版年：2011
• 出版时间：October 6, 2011
• 年：2011
• 卷：115
• 期：39
• 页码：19136-19140
• 全文大小：807K
• 年卷期：v.115,no.39(October 6, 2011)
• ISSN：1932-7455

文摘

Control of supramolecular architectures inherent in layer-by-layer (LbL) films allows for synergistic effects between the film components, as in reactions under controlled conditions. In this paper we show that a bienzyme system can be produced in LbL films, in which the product of a reaction catalyzed by invertase (INV) is used in a catalysis process involving glucose oxidase (GOx). As a proof of principle, simultaneous detection of glucose and sucrose is demonstrated for an optimized film architecture comprising poly allyllamine hydrochloride (PAH) alternated with INV + GOx layers (PAH/(GOx + INV))₁₀. Though the sensitivity for sucrose is low because the product from the reaction with invertase has low electroactivity, the results confirm that cascade reactions can be performed in a controlled manner in LbL films with possible applications in sensors and bioelectrochemical devices.