Development and Certification of a Standard Reference Material for Vitamin D Metabolites in Human Serum

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• 作者：Karen W. Phinney ; Mary Bedner ; Susan S.-C. Tai ; Veronica V. Vamathevan ; Lane C. Sander ; Katherine E. Sharpless ; Stephen A. Wise ; James H. Yen ; Rosemary L. Schleicher ; Madhulika Chaudhary-Webb ; Christine M. Pfeiffer ; Joseph M. Betz ; Paul M. Coates ; Mary Frances Picciano
• 刊名：Analytical Chemistry
• 出版年：2012
• 出版时间：January 17, 2012
• 年：2012
• 卷：84
• 期：2
• 页码：956-962
• 全文大小：230K
• 年卷期：v.84,no.2(January 17, 2012)
• ISSN：1520-6882

文摘

The National Institute of Standards and Technology (NIST), in collaboration with the National Institutes of Health鈥檚 Office of Dietary Supplements (NIH-ODS), has developed a Standard Reference Material (SRM) for the determination of 25-hydroxyvitamin D [25(OH)D] in serum. SRM 972 Vitamin D in Human Serum consists of four serum pools with different levels of vitamin D metabolites and has certified and reference values for 25(OH)D₂, 25(OH)D₃, and 3-epi-25(OH)D₃. Value assignment of this SRM was accomplished using a combination of three isotope-dilution mass spectrometry approaches, with measurements performed at NIST and at the Centers for Disease Control and Prevention (CDC). Chromatographic resolution of the 3-epimer of 25(OH)D₃ proved to be essential for accurate determination of the metabolites.