Targeted Metabolite Analysis and Antioxidant Potential of Rumex induratus
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- 作者：Luí ; s Guerra ; Clara Pereira ; Paula B. Andrade ; M. Â ; ngelo Rodrigues ; Federico Ferreres ; Paula Guedes De Pinho ; Rosa M. Seabra ; Patrí ; cia Valentã ; o
- 刊名：Journal of Agricultural and Food Chemistry
- 出版年：2008
- 出版时间：September 10, 2008
- 年：2008
- 卷：56
- 期：17
- 页码：8184-8194
- 全文大小：226K
- 年卷期：v.56,no.17(September 10, 2008)
- ISSN：1520-5118
文摘
Targeted metabolite analysis of aqueous extract of Rumex induratus leaves, in terms of phenolic compounds and organic acids, and the study of its antioxidant activity against the DPPH• radical, a reactive oxygen species, hypochlorous acid, and a reactive nitrogen species, nitric oxide (•NO), were performed. The samples were collected in several locations, spontaneously occurring or from greenhouse culture, at different stages of development and seasons. The phenolic composition was achieved by high-performance liquid chromatography (HPLC)−diode array detection, and four hydroxycinnamic acid derivatives and 10 flavonoid glycosides (C- and O-heterosides) were determined. Organic acids composition was established by HPLC-UV, revealing five compounds. The total amount of phenolic compounds and organic acids were affected by growing conditions and developmental phase. The aqueous extract exhibited a dose-related activity against all tested reactive species.