IMS-IMS and IMS-IMS-IMS/MS for Separating Peptide and Protein Fragment Ions

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• 作者：Samuel I. Merenbloom ; Stormy L. Koeniger ; Stephen J. Valentine ; Manolo D. Plasencia ; and David E. Clemmer
• 刊名：Analytical Chemistry
• 出版年：2006
• 出版时间：April 15, 2006
• 年：2006
• 卷：78
• 期：8
• 页码：2802 - 2809
• 全文大小：614K
• 年卷期：v.78,no.8(April 15, 2006)
• ISSN：1520-6882

文摘

Multidimensional ion mobility spectrometry (IMS-IMSand IMS-IMS-IMS) techniques have been combinedwith mass spectrometry (MS) and investigated as a meansof generating and separating peptide and protein fragmentions. When fragments are generated inside a drift tubeand then dispersed by IMS prior to MS analysis, it ispossible to observe many features that are not apparentfrom MS analysis alone. The approach is demonstratedby examining fragmentation patterns arising from electrospray ion distributions of insulin chain B and ubiquitin.The multidimensional IMS approach makes it possible toselect individual components for collisional activation andto disperse fragments based on differences in mobilityprior to MS analysis. Such an approach makes it possibleto observe many features not apparent by MS analysisalone.