In Vivo and In Vitro Debromination of Decabromodiphenyl Ether (BDE 209) by Juvenile Rainbow Trout and Common Carp

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• 作者：Heather M. Stapleton ; Brian Brazil ; R. David Holbrook ; Carys L. Mitchelmore ; Rae Benedict ; Alex Konstantinov ; Dave Potter
• 刊名：Environmental Science & Technology
• 出版年：2006
• 出版时间：August 1, 2006
• 年：2006
• 卷：40
• 期：15
• 页码：4653 - 4658
• 全文大小：189K
• 年卷期：v.40,no.15(August 1, 2006)
• ISSN：1520-5851

文摘

Decabromodiphenyl ether (BDE 209), the major congenerin the high volume industrial flame retardant mixture"DecaBDE", has recently been shown to be metabolizedby carp. To further explore this phenomenon, juvenile rainbowtrout were exposed to BDE 209 via the diet for a fivemonth period. Analysis of the whole body homogenate,liver, serum, and intestinal tissues revealed that BDE 209accumulated in rainbow trout tissues and was mostconcentrated in the liver. In addition to BDE 209, severalhepta-, octa-, and nonaBDE congeners also accumulated inrainbow trout tissues over the same period as a resultof BDE 209 debromination. Based on the total body burdenof the hepta- through decaBDE congeners, uptake ofBDE 209 was estimated at 3.2%. Congener profiles weredifferent among whole body homogenate, liver, and serum,with the whole body homogenates having a greatercontribution of the debrominated biotransformation products.Extracts of the rainbow trout whole body homogenateswere compared with extracts from a previous experimentwith common carp. This comparison revealed that BDE202 (2,2',3,3',5,5',6,6'-octabromodiphenyl ether) was a dominantdebromination product in both studies. To determinewhether the observed debromination was metabolicallydriven, liver microsomal fractions were prepared from bothcommon carp and rainbow trout. Analysis of the microsomalfractions following incubation with BDE 209 revealedthat rainbow trout biotransformed as much as 22% of theBDE 209 mass, primarily to octa- and nonaBDE congeners.In contrast, carp liver microsomes biotransformed up to65% of the BDE 209 mass, primarily down to hexaBDEcongeners. These microsomal incubations confirm a metabolicpathway for BDE 209 debromination.