Hu
man
milk oligosaccharides (HMOs), as an abundant and bioactive co
mponent of breast
milk, work in
many ways to pro
mote the health of breast fed infants. The expression of HMOs has been shown to vary in accordance with Lewis blood type and secretor status, as wo
men of different blood types differ in the expression of 伪1,2 fucosyltransferase (FUT2) and 伪1,3/4 fucosyltransferase (FUT3). In this study, HMOs were extracted fro
m the
milk of 60 wo
men fro
m The Ga
mbia, Africa with various Lewis and secretor blood types. The HMOs were profiled using high resolution HPLC-Chip/TOF
mass spectro
metry. Notably, the a
mounts of fucosylation varied significantly between Le(a+b-) nonsecretors, Le(a-b+) and Le(a-b-) secretors, and Le(a-b-) nonsecretors. With higher frequency of expression of the recessive Lewis negative and nonsecretor phenotypes in West African populations, the HMO profiles of several
milks fro
m wo
men of these phenotypes were exa
mined, de
monstrating decreased a
mounts of total oligosaccharide abundance and lower relative a
mounts of fucosylation. Also in this study, four specific fucosylated structures (2鈥睩L, LNFP I, LDFT, and LNDFH I) were deter
mined to be specific and sensitive glycan
markers for rapidly deter
mining secretor status without the need for serological testing.
Keywords:
Lewis blood type; secretor status; man+milk+oligosaccharides&qsSearchArea=searchText">human milk oligosaccharides; nano LC-TOF/MS