Antibody-Conjugated CdTe Quantum Dots for Escherichia coli Detection

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• 作者：Yu-Ching Kuo ; Qiang Wang ; Chada Ruengruglikit ; Hailong Yu ; Qingrong Huang
• 刊名：Journal of Physical Chemistry C
• 出版年：2008
• 出版时间：April 3, 2008
• 年：2008
• 卷：112
• 期：13
• 页码：4818 - 4824
• 全文大小：281K
• 年卷期：v.112,no.13(April 3, 2008)
• ISSN：1932-7455

文摘

Chemically denatured bovine serum albumin (dBSA)-coated water-soluble cadmium telluride (CdTe) quantumdots (QDs), which can effectively improve the chemical stability and photoluminescence quantum yield ofCdTe QDs, were successfully conjugated to an anti-Escherichia coli antibody via a cross-linking reaction.The formation of antibody-conjugated CdTe QDs was confirmed using gel filtration chromatography. Theanti-E. coli antibody-conjugated CdTe QDs were then used to detect E. coli O157:H7 and Listeriamonocytogenes using fluorescence microscopy. It was shown that, after being conjugated to CdTe QDs, theanti-E. coli antibody still maintained its bioactivity and biorecognition specificity. Succinylated dBSA-coatedCdTe QDs also were prepared to conjugate with the anti-E. coli antibody. However, no evidence was foundthat the succinylation provided a better route for antibody conjugation. Our results demonstrate the potentialof bioconjugated CdTe QDs for broad biological applications, such as fluorescence-based pathogen detectionand in vitro or in vivo cell imaging.