Suppression of Bacterial Wilt of Tomato by Bioorganic Fertilizer Made from the Antibacterial Compound Producing Strain Bacillus amyloliquefaciens HR62
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- 作者：Jianfeng Huang ; Zhong Wei ; Shiyong Tan ; Xinlan Mei ; Qirong Shen ; Yangchun Xu
- 刊名：Journal of Agricultural and Food Chemistry
- 出版年：2014
- 出版时间：November 5, 2014
- 年：2014
- 卷：62
- 期：44
- 页码：10708-10716
- 全文大小：453K
- ISSN：1520-5118
文摘
Ralstonia solanacearum (Smith) is an important soil-borne pathogen worldwide. We investigated the effects of a new bioorganic fertilizer, BIO62, which was made from organic fertilizer and antagonist Bacillus amyloliquefaciens HR62, on the control of bacterial wilt of tomato in greenhouse condition. The results showed that the application of BIO62 significantly decreased disease incidence by 65% and strongly reduced R. solanacearum populations both in the rhizosphere soil (8.04 log cfu g鈥? dry soil) and crown sections (5.63 log cfu g鈥? fresh plant section) at 28 days after pathogen challenge. Antibacterial compounds produced by HR62 were purified by silica gel, Sephadex LH-20, and HPLC and then identified using HPLC/electrospray ionization mass spectrometry analysis. Macrolactin A and 7-O-malonyl macrolactin A (molecular weights of 402 and 488 Da, respectively), along with surfactin B (molecular weights of 994, 1008, 1022, and 1036 Da), were observed to inhibit the growth of R. solanacearum.