Design, Synthesis, and Incorporation of Fluorous 5-Methylcytosines into Oligonucleotides

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• 作者：Zhiquan Song ; Qisheng Zhang
• 刊名：Journal of Organic Chemistry
• 出版年：2011
• 出版时间：December 16, 2011
• 年：2011
• 卷：76
• 期：24
• 页码：10263-10268
• 全文大小：306K
• 年卷期：v.76,no.24(December 16, 2011)
• ISSN：1520-6904

文摘

A palladium-catalyzed Negishi coupling reaction has been developed to synthesize fluorous 5-methylcytosines. These fluorous nucleosides are incorporated into the oligonucleotides that correspond to part of the promoter region of Oct4, a master gene that undergoes dynamic DNA demethylation during cellular reprogramming. The separation of the fluorous oligonucleotides from its nonfluorous analogues has been achieved through solid-phase extraction over fluorous silica, suggesting its potential use in probing DNA demethylation.