Resolution-Enhanced 4D 15N/13C NOESY Protein NMR Spectroscopy by Application of the Covariance Transform

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• 作者：David A. Snyder ; Yingqi Xu ; Daiwen Yang ; Rafael Br&uuml ; schweiler
• 刊名：Journal of the American Chemical Society
• 出版年：2007
• 出版时间：November 21, 2007
• 年：2007
• 卷：129
• 期：46
• 页码：14126 - 14127
• 全文大小：50K
• 年卷期：v.129,no.46(November 21, 2007)
• ISSN：1520-5126

文摘

The combination of shared-evolution 4D ¹⁵N/¹³C-edited NOESY spectroscopy with covariance NMR is introduced, which yields, as a sub-spectrum, an asymmetric 4D ¹⁵N/¹³C-edited NOESY spectrum at a resolution enhanced 5-fold over the one of a non-shared 4D ¹⁵N/¹³C-edited NOESY measured with the same sweep widths and number of increments. The achieved resolution enhancement allows for a substantial increase in the number of assigned NOEs over that of the 4D Fourier transform spectrum and should prove useful for efficient, high-resolution NMR-based studies of protein structure.