Design and Construction of Double-Decker Tile as a Route to Three-Dimensional Periodic Assembly of DNA

详细信息    查看全文

• 作者：Urmi Majumder ; Abhijit Rangnekar ; Kurt V. Gothelf ; John H. Reif ; Thomas H. LaBean
• 刊名：Journal of the American Chemical Society
• 出版年：2011
• 出版时间：March 23, 2011
• 年：2011
• 卷：133
• 期：11
• 页码：3843-3845
• 全文大小：717K
• 年卷期：v.133,no.11(March 23, 2011)
• ISSN：1520-5126

文摘

DNA is a useful material for nanoscale construction. Due to highly specific Watson鈭扖rick base pairing, the DNA sequences can be designed to form small tiles or origami. Adjacent helices in such nanostructures are connected via Holliday junction-like crossovers. DNA tiles can have sticky ends which can then be programmed to form large one-dimensional and two-dimensional periodic lattices. Recently, a three-dimensional DNA lattice has also been constructed. Here we report the design and construction of a novel DNA cross tile, called the double-decker tile. Its arms are symmetric and have four double helices each. Using its sticky ends, large two-dimensional square lattices have been constructed which are on the order of tens of micrometers. Furthermore, it is proposed that the sticky ends of the double-decker tile can be programmed to form a three-dimensional periodic lattice with large cavities that could be used as a scaffold for precise positioning of molecules in space.