A Product of Ozonolysis of Cholesterol Alters the Biophysical Properties of Phosphatidylethanolamine Membranes
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- 作者：Ellen Wachtel ; Diana Bach ; Raquel F. Epand ; Arye Tishbee ; and Richard M. Epand
- 刊名：Biochemistry
- 出版年：2006
- 出版时间：January 31, 2006
- 年：2006
- 卷：45
- 期：4
- 页码：1345 - 1351
- 全文大小：121K
- 年卷期：v.45,no.4(January 31, 2006)
- ISSN：1520-4995
文摘
There is evidence that some products of the reaction of ozone with cholesterol contribute toatherosclerosis. One of these compounds is 3
fchars/beta2.gif" BORDER=0 ALIGN="middle">-hydroxy-5-oxo-5,6-secocholestan-6-al. We have synthesizedthis compound and have demonstrated that it reacts with phosphatidylethanolamine to form a Schiff base.The 3fchars/beta2.gif" BORDER=0 ALIGN="middle">-hydroxy-5-oxo-5,6-secocholestan-6-al also affects the physical properties of phosphatidylethanolamines. We show by both DSC and X-ray diffraction that it increases the negative curvature of themembrane. In addition, 3fchars/beta2.gif" BORDER=0 ALIGN="middle">-hydroxy-5-oxo-5,6-secocholestan-6-al causes the lamellar phase to becomedisorganized, resulting in the loss of lamellar periodicity. The chemical and physical interactions of 3fchars/beta2.gif" BORDER=0 ALIGN="middle">-hydroxy-5-oxo-5,6-secocholestan-6-al with phosphatidylethanolamines may contribute to damaging effectsof this lipid on cell membranes, resulting in pathology.
fchars/beta2.gif" BORDER=0 ALIGN="middle">-hydroxy-5-oxo-5,6-secocholestan-6-al. We have synthesizedthis compound and have demonstrated that it reacts with phosphatidylethanolamine to form a Schiff base.The 3fchars/beta2.gif" BORDER=0 ALIGN="middle">-hydroxy-5-oxo-5,6-secocholestan-6-al also affects the physical properties of phosphatidylethanolamines. We show by both DSC and X-ray diffraction that it increases the negative curvature of themembrane. In addition, 3fchars/beta2.gif" BORDER=0 ALIGN="middle">-hydroxy-5-oxo-5,6-secocholestan-6-al causes the lamellar phase to becomedisorganized, resulting in the loss of lamellar periodicity. The chemical and physical interactions of 3fchars/beta2.gif" BORDER=0 ALIGN="middle">-hydroxy-5-oxo-5,6-secocholestan-6-al with phosphatidylethanolamines may contribute to damaging effectsof this lipid on cell membranes, resulting in pathology.