Behavior of Polycatalytic Assemblies in a Substrate-Displaying Matrix

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• 作者：Renjun Pei ; Steven K. Taylor ; Darko Stefanovic ; Sergei Rudchenko ; Tiffany E. Mitchell ; Milan N. Stojanovic
• 刊名：Journal of the American Chemical Society
• 出版年：2006
• 出版时间：October 4, 2006
• 年：2006
• 卷：128
• 期：39
• 页码：12693 - 12699
• 全文大小：249K
• 年卷期：v.128,no.39(October 4, 2006)
• ISSN：1520-5126

文摘

We describe polycatalytic assemblies, comprising one or two streptavidin molecules and two tosix attached nucleic acid catalysts (deoxyribozymes), with phosphodiesterase activity. When exposed to amatrix covered at high densities with oligonucleotide substrates, these molecules diffuse through the matrixcontinuously cleaving the substrate at rates comparable to those of individual catalysts in solution. Ratesof diffusion (movement), processivity, and resident times of assemblies can be controlled through the numberof catalytic units and the length of substrate/product recognition regions. The assemblies were characterizedat the ensemble level using surface plasmon resonance.