Luminescent Alkyne-Bearing Terbium(III) Complexes and Their Application to Bioorthogonal Protein Labeling

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• 作者：William I. O’Malley ; Elwy H. Abdelkader ; Margaret L. Aulsebrook ; Riccardo Rubbiani ; Choy-Theng Loh ; Michael R. Grace ; Leone Spiccia ; Gilles Gasser ; Gottfried Otting ; Kellie L. Tuck ; Bim Graham
• 刊名：Inorganic Chemistry
• 出版年：2016
• 出版时间：February 15, 2016
• 年：2016
• 卷：55
• 期：4
• 页码：1674-1682
• 全文大小：511K
• ISSN：1520-510X

文摘

Two new bifunctional macrocyclic chelate ligands that form luminescent terbium(III) complexes featuring an alkyne group for conjugation to (bio)molecules via the Cu(I)-catalyzed “click” reaction were synthesized. Upon ligation, the complexes exhibit a significant luminescent enhancement when excited at the λ_max of the “clicked” products. To demonstrate the utility of the complexes for luminescent labeling, they were conjugated in vitro to E. coli aspartate/glutamate-binding protein incorporating a genetically encoded p-azido-l-phenylalanine or p-(azidomethyl)-l-phenylalanine residue. The complexes may prove useful for time-gated assay applications.