The origin of the unusual regioselectivity of heme oxygenation, i.e. the oxidation of heme to
![](/images/gifchars/delta.gif)
-biliverdin (70%) and
![](/images/gifchars/beta2.gif)
-biliverdin (30%), that is exhibited by heme oxygenase from
Pseudomonasaeruginosa (
pa-HO) has been studied by
1H NMR,
13C NMR, and resonance Raman spectroscopies.Whereas resonance Raman indicates that the heme-iron ligation in
pa-HO is homologous to that observedin previously studied
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-hydroxylating heme oxygenases, the NMR spectroscopic studies suggest that theheme in this enzyme is seated in a manner that is distinct from that observed for all other
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-hydroxylatingheme oxygenase enzymes for which a structure is known. In
pa-HO, the heme is rotated in-plane ~110
![](/images/entities/deg.gif)
,so the
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-meso-carbon of the major orientational isomer is located within the HO-fold in the place where the
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-hydroxylating enzymes typically place the
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-meso-carbon. The unusual heme seating displayed by
pa-HO places the heme propionates so that these groups point in the direction of the solvent-exposed hemeedge and appears to originate in large part from the absence of stabilizing interactions between thepolypeptide and the heme propionates, which are typically found in
![](/images/gifchars/alpha.gif)
-hydroxylating heme oxygenaseenzymes. These interactions typically involve Lys-16 and Tyr-112, in
Neisseriae meningitidis HO, and Lys-16 and Tyr-134, in human and rat HO-1. The corresponding residues in
pa-HO are Asn-19 and Phe-117,respectively. In agreement with this hypothesis, we found that the Asn-19 Lys/Phe-117 Tyr double mutantof
pa-HO exists as a mixture of molecules exhibiting two distinct heme seatings; one seating is identical tothat exhibited by wild-type
pa-HO, whereas the alternative seating is very similar to that typical of
![](/images/gifchars/alpha.gif)
-hydroxylating heme oxygenase enzymes and is related to the wild-type seating by ~110
![](/images/entities/deg.gif)
in-plane rotationof the heme. Furthermore, each of these heme seatings in the
pa-HO double mutant gives rise to a subsetof two heme isomeric orientations that are related to each other by 180
![](/images/entities/deg.gif)
rotation about the
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-
![](/images/gifchars/gamma.gif)
-meso-axis. The coexistence of these molecules in solution, in the proportions suggested by the correspondingarea under the peaks in the
1H NMR spectrum, explains the unusual regioselectivity of heme oxygenationobserved with the double mutant, which we found produces
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- (55%),
![](/images/gifchars/delta.gif)
- (35%), and
![](/images/gifchars/beta2.gif)
-biliverdin (10%).
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-Biliverdin is obtained by oxidation of the heme seated similar to that of
![](/images/gifchars/alpha.gif)
-hydroxylating enzymes, whereas
![](/images/gifchars/beta2.gif)
- and
![](/images/gifchars/delta.gif)
-biliverdin are formed from the oxidation of heme seated as in wild-type
pa-HO.