Designed Signaling Aptamers that Transduce Molecular Recognition to Changes in Fluorescence Intensity

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• 作者：Sulay D. Jhaveri ; Romy Kirby ; Rick Conrad ; Emily J. Maglott ; Michael Bowser ; Robert T. Kennedy ; Gary Glick ; and Andrew D. Ellington
• 刊名：Journal of the American Chemical Society
• 出版年：2000
• 出版时间：March 22, 2000
• 年：2000
• 卷：122
• 期：11
• 页码：2469 - 2473
• 全文大小：315K
• 年卷期：v.122,no.11(March 22, 2000)
• ISSN：1520-5126

文摘

We have engineered aptamers that contain fluorescent reporters and that signal the presence ofcognate ligands in solution. Two different anti-adenosine "signaling aptamers", one made from RNA and onefrom DNA, can selectively signal the presence of adenosine in solution. Increases in fluorescence intensityreproducibly follow increases in adenosine concentration, and can be used for quantitation. The facile methodswe have developed can potentially be used for generating a wide variety of signaling aptamers for use insensor arrays.