Quantum Dots Enhanced Ultrasensitive Detection of DNA Adducts

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• 作者：Zhixin Wang ; Meiling Lu ; Xiaoli Wang ; Ruichuan Yin ; Yuling Song ; X. Chris Le ; Hailin Wang
• 刊名：Analytical Chemistry
• 出版年：2009
• 出版时间：December 15, 2009
• 年：2009
• 卷：81
• 期：24
• 页码：10285-10289
• 全文大小：240K
• 年卷期：v.81,no.24(December 15, 2009)
• ISSN：1520-6882

文摘

Here we demonstrate that quantum dots (QD) can greatly improve the ultrasensitive capillary electrophoresis-laser induced fluorescence immunoassay of trace anti-benzo(a)pyrene diol epoxide (BPDE)−DNA adducts from sensitivity to separation. We for the first time show that the target QD−antibody−DNA complex is not only effectively separated but also effectively focused by capillary electrophoresis. With the online laser-induced fluorescence detection coupled, the low limits of detection of 6.6 × 10⁻²¹ mol in mass and 120 fM in concentration are achieved for BPDE−DNA adducts. The achieved ultrasensitivity allows for human exposure biomonitoring and shows promising applications of QD in various DNA analyses, including DNA damage.