Morinda citrifolia L. (Rubiaceae), known as noni, has a long history of traditional use in the Hawaiianand Tahitian islands. More recently, an array of commercial noni fruit juice products are gainingpopularity as dietary supplements, with claims of anticancer and immunostimulant activities. Thebiologically active principles of noni are not fully known. In continuation of work on the isolation ofmarkers from dietary supplements, this paper reports the isolation of three new markers, namely,1-
O-(3'-methylbut-3'-enyl)-
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-
D-glucopyranose (
1), 1-
n-butyl-4-(5'-formyl-2'-furanyl)methyl succinate(
2), and 4-
epi-borreriagenin (
3), together with the known iridoid glycosides asperulosidic acid (
4) anddeacetylasperulosidic acid (
5) and a mixture of 1-
n-butyl-4-methyl-2-hydroxysuccinate (
6a) and 1-
n-butyl-4-methyl-3-hydroxysuccinate (
6b), as well as a mixture of
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- and
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-glucopyranose from nonifruit juice obtained from Puerto Rico. The structures of compounds were based on
1H and
13C NMR,mainly 2D NMR COSY, HMQC, HMBC, and NOESY experiments, and HRMS. Furthermore, samplesfrom fresh-squeezed noni fruit juice from Japan revealed the presence of scopoletin (
7), in additionto compounds
1-
6, indicating no significant differences in the marker constituents of noni collectedfrom Atlantic and Pacific regions.