Variability in C3-Plant Cell-Wall Biosynthesis in a High-CO2 Atmosphere by Solid-State NMR Spectroscopy

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• 作者：Tsyr-Yan Yu ; Manmilan Singh ; Shigeru Matsuoka ; Gary J. Patti ; Gregory S. Potter ; Jacob Schaefer
• 刊名：Journal of the American Chemical Society
• 出版年：2010
• 出版时间：May 12, 2010
• 年：2010
• 卷：132
• 期：18
• 页码：6335-6341
• 全文大小：256K
• 年卷期：v.132,no.18(May 12, 2010)
• ISSN：1520-5126

文摘

We have used a frequency-selective rotational-echo double-resonance (REDOR) solid-state NMR experiment to measure the concentrations of glycine−glycine pairs in proteins (and protein precursors) of intact leaves of plants exposed to both high- and low-CO₂ atomospheres. The results are interpreted in terms of differences in cell-wall biosynthesis between plant species. We illustrate this variability by comparing the assimilation of label in cheatgrass and soybean leaves labeled using ¹⁵N-fertilizer and ¹³CO₂ atmospheres. Cheatgrass and soybean are both C₃ plants but differ in their response to a high-CO₂ environment. Based on REDOR results, we determined that cheatgrass (a plant that seems likely to flourish in future low-water, high-CO₂ environments) routes 2% of the assimilated carbon label that remains in the leaf after 1 h in a 600-ppm ¹³CO₂ atmosphere to glycine-rich protein (or its precursors), a structural component of cell walls cross-linked to lignins. In contrast, soybean under the same conditions routes none of its assimilated carbon to glycine-rich protein.