An Innovative Method To Study Target Protein−Drug Interactions by Mass Spectrometry
详细信息 查看全文
- 作者：Mathias Q. Mller ; Leo J. de Koning ; Andreas Schmidt ; Christian Ihling ; Yvonne Syha ; Oliver Rau ; Karl Mechtler ; Manfred Schubert-Zsilavecz ; Andrea Sinz
- 刊名：Journal of Medicinal Chemistry
- 出版年：2009
- 出版时间：May 14, 2009
- 年：2009
- 卷：52
- 期：9
- 页码：2875-2879
- 全文大小：180K
- 年卷期：v.52,no.9(May 14, 2009)
- ISSN：1520-4804
文摘
We report the combination of chemical cross-linking and high-resolution mass spectrometry for analyzing conformational changes in target proteins that are induced by drug binding. With this approach conformational changes in the peroxisome proliferator-activated receptor alpha (PPARα) upon binding of low-molecular weight compounds were readily detected, proving that the strategy provides a basis to efficiently characterize target protein−drug interactions.