General Peroxidase Activity of G-Quadruplex−Hemin Complexes and Its Application in Ligand Screening

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• 作者：Xiaohong Cheng ; Xiangjun Liu ; Tao Bing ; Zehui Cao ; Dihua Shangguan
• 刊名：Biochemistry
• 出版年：2009
• 出版时间：August 25, 2009
• 年：2009
• 卷：48
• 期：33
• 页码：7817-7823
• 全文大小：867K
• 年卷期：v.48,no.33(August 25, 2009)
• ISSN：1520-4995

文摘

DNA sequences with repetitive G-rich structural motifs, which form special structures called G-quadruplexes, widely exist in the human genome. Here we report the general peroxidase activity of G-quadruplex−hemin complexes and discuss the connection between peroxidase activity and G-quadruplex structures. The high peroxidase activity of hemin complexed with intramolecular parallel G-quadruplex-forming sequences in gene promoters (such as c-Myc, VEGF, c-Kit21, HIF-1α, and RET) may imply a potential mechanism of hemin-mediated cellular injury. This peroxidase activity has also been demonstrated to be applicable for screening G-quadruplex ligands (potential anticancer reagents) using colorimetric and visual detection strategies.