Selective Enhancement of Carbohydrate Ion Abundances by Diamond Nanoparticles for Mass Spectrometric Analysis

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• 作者：Chieh-Lin Wu ; Chia-Chen Wang ; Yin-Hung Lai ; Hsun Lee ; Jia-Der Lin ; Yuan Tseh Lee ; Yi-Sheng Wang
• 刊名：Analytical Chemistry
• 出版年：2013
• 出版时间：April 16, 2013
• 年：2013
• 卷：85
• 期：8
• 页码：3836-3841
• 全文大小：398K
• 年卷期：v.85,no.8(April 16, 2013)
• ISSN：1520-6882

文摘

Diamond nanoparticles (DNPs) were incorporated into matrix-assisted laser desorption/ionization (MALDI) samples to enhance the sensitivity of the mass spectrometer to carbohydrates. The DNPs optimize the MALDI sample morphology and thermalize the samples for thermally labile compounds because they have a high thermal conductivity, a low extinction coefficient in UV鈥搗is spectral range, and stable chemical properties. The best enhancement effect was achieved when matrix, DNP, and carbohydrate solutions were deposited and vacuum-dried consecutively to form a trilayer sample morphology. It allows the direct identification of underivatized carbohydrates mixed with equal amount of proteins because no increase in the ion abundance of proteins was achieved. For dextran with an average molecular weight of 1500, the trilayer method typically improves the sensitivity by 79- and 7-fold in comparison to the conventional dried-droplet and thin-layer methods, respectively.