Application of an Absorption-Based Surface Plasmon Resonance Principle to the Development of SPR Ammonium Ion and Enzyme Sensors
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- 作者：Eiji Fujii ; Takashi Koike ; Kaori Nakamura ; Shin-ichi Sasaki ; Kazuyoshi Kurihara ; Daniel Citterio ; Yuzuru Iwasaki ; Osamu Niwa ; and Koji Suzuki
- 刊名：Analytical Chemistry
- 出版年：2002
- 出版时间：December 1, 2002
- 年：2002
- 卷：74
- 期：23
- 页码：6106 - 6110
- 全文大小：111K
- 年卷期：v.74,no.23(December 1, 2002)
- ISSN：1520-6882
文摘
Two new types of surface plasmon resonance (SPR)sensors that can determine the concentration of ammonium cations and urea were realized based on thepreviously reported theory of the absorption-based SPRmeasurement method. The change of the dielectric constant caused by the change of the light absorption characteristics of dyes incorporated in a sensing membranephase is utilized in these SPR sensors. The determinationof ions using the SPR sensor was realized by detectingthe SPR signals of the minimum reflectance related to thechange of absorption spectra of the dye in the ion optodemembrane consisting of an ammonium-selective ionophore (TD19C6) and a lipophilic cationic dye (KD-M11)that shows absorption spectral changes due to protonationand deprotonation. A SPR enzyme sensor that can determine the concentration of urea was prepared by thecombination of this ion optode membrane and an enzymemembrane based on urease. With the newly developedSPR sensors, the intensity changes of the reflectance atthe fixed SPR resonance angle are monitored, which isdifferent from conventional SPR sensors where usuallythe change of the SPR resonance angles is detected. In acontinuous-flow experiment using the SPR ion sensor forNH4+ ion determination, a dynamic measurement rangefrom 10-5 to 10-2 M was achieved. In the case of theenzyme-based SPR urea sensor, a dynamic range from10-4 to 10-1 M was observed in a stopped-flow batcharrangement. It is expected that this sensing techniquecan be applied for the SPR-based detection of a wide rangeof low molecular weight analytes.