Affinity-Driven Immobilization of Proteins to Hematite Nanoparticles

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• 作者：Elaheh Zare-Eelanjegh ; Debajeet K. Bora ; Patrick Rupper ; Krisztina Schrantz ; Linda Thöny-Meyer ; Katharina Maniura-Weber ; Michael Richter ; Greta Faccio
• 刊名：ACS Applied Materials & Interfaces
• 出版年：2016
• 出版时间：August 10, 2016
• 年：2016
• 卷：8
• 期：31
• 页码：20432-20439
• 全文大小：420K
• 年卷期：0
• ISSN：1944-8252

文摘

Functional nanoparticles are valuable materials for energy production, bioelectronics, and diagnostic devices. The combination of biomolecules with nanosized material produces a new hybrid material with properties that can exceed the ones of the single components. Hematite is a widely available material that has found application in various sectors such as in sensing and solar energy production. We report a single-step immobilization process based on affinity and achieved by genetically engineering the protein of interest to carry a hematite-binding peptide. Fabricated hematite nanoparticles were then investigated for the immobilization of the two biomolecules C-phycocyanin (CPC) and laccase from Bacillus pumilus (LACC) under mild conditions. Genetic engineering of biomolecules with a hematite-affinity peptide led to a higher extent of protein immobilization and enhanced the catalytic activity of the enzyme.