Identification and Characterization of FabA from the Type II Fatty Acid Synthase of Streptomyces coelicolor
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  • 作者:Renu Singh ; Kevin A. Reynolds
  • 刊名:Journal of Natural Products
  • 出版年:2016
  • 出版时间:January 22, 2016
  • 年:2016
  • 卷:79
  • 期:1
  • 页码:240-243
  • 全文大小:273K
  • ISSN:1520-6025
文摘
FabA is proposed to catalyze the dehydration step of chain elongation in fatty acid and undecylprodiginine biosynthesis in Streptomyces coelicolor. Analysis of the S. coelicolor genome has revealed a fabA gene (SCO4636-SCO4637, encoding a heterodimer 3-hydroxyacyl-ACP dehydratase). Herein, we report the identification and characterization of the corresponding gene products. Kinetic analysis has demonstrated that FabA is capable of utilizing various chain lengths of straight- and branched-chain 3-hydroxyacyl-NAC substrates. Additionally, FabA does not discriminate between acyl carrier proteins (ACPs) from primary and secondary metabolism. These data provide the first experimental evidence that FabA has 3-hydroxyacyl-ACP dehydratase activity and processes intermediates for both biosynthetic pathways.

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