Functional Compartmental Modeling of the Photosystems in the Thylakoid Membrane at 77 K

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• 作者：Joris J. Snellenburg ; Jan P. Dekker ; Rienk van Grondelle ; Ivo H. M. van Stokkum
• 刊名：Journal of Physical Chemistry B
• 出版年：2013
• 出版时间：September 26, 2013
• 年：2013
• 卷：117
• 期：38
• 页码：11363-11371
• 全文大小：429K
• 年卷期：v.117,no.38(September 26, 2013)
• ISSN：1520-5207

文摘

Time-resolved fluorescence spectroscopy measurements at 77 K on thylakoid membrane preparations and isolated photosynthetic complexes thereof were investigated using target analysis with the aim of building functional compartmental models for the photosystems in the thylakoid membrane. Combining kinetic schemes with different spectral constraints enabled us to resolve the energy transfer pathways and decay characteristics of the different emissive species. We determined the spectral and energetic properties of the red Chl pools in both photosystems and quantified the formation of LHCII-LHCI-PSI supercomplexes in the transition from native to unstacked thylakoid membranes.