Multiplexed Cell Signaling Analysis of Human Breast Cancer Applications for Personalized Therapy
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- 作者：Julia D. Wulfkuhle ; Runa Speer ; Mariaelena Pierobon ; Julie Laird ; Virginia Espina ; Jianghong Deng ; Enzo Mammano ; Sherry X. Yang ; Sandra M. Swain ; Donato Nitti ; Laura J. Esserman ; Claudio Belluco ; Lanc
- 刊名：Journal of Proteome Research
- 出版年：2008
- 出版时间：April 2008
- 年：2008
- 卷：7
- 期：4
- 页码：1508 - 1517
- 全文大小：10052K
- 年卷期：v.7,no.4(April 2008)
- ISSN：1535-3907
文摘
Phosphoprotein driven cellular signaling events represent most of the new molecular targets for cancer treatment. Application of reverse-phase protein microarray technology for the study of ongoing signaling activity within breast tumor specimens holds great potential for elucidating and profiling signaling activity in real-time for patient-tailored therapy. Analysis of laser capture microdissection primary human breast tumors and metastatic lesions reveals pathway specific profiles and a new way to classify cancer based on functional signaling portraits. Moreover, the data demonstrate the requirement of laser capture microdissection for analysis and reveal the metastasis-specific changes that occur within a new microenvironment. Analysis of biopsy material from clinical trials for targeted therapeutics demonstrates the feasibility and utility of comprehensive signal pathway activation profiling for molecular analysis.